Hotspot

rpoA

LSC · 74513-75523 · 1011 bp

Back to Tetraena

Pi 0.0642

MarkerSeek score 80.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1011
variable_sites: 95
pi: 0.0642
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CATTTCGTCGTCCAGTAGCA	GCAGGAAATGCGATTCGAAC	50.0	50.0	58.0	58.2	1517-1533 bp	1.0	86.8
rpoA_p2	TAATGGTTTCGGTACCTGCG	GCAGGAAATGCGATTCGAAC	50.0	50.0	58.0	58.2	1586-1602 bp	1.0	86.7
rpoA_p3	TTATGGAGAACCTGCATGGC	GCAGGAAATGCGATTCGAAC	50.0	50.0	57.9	58.2	1807-1823 bp	1.0	85.9
rpoA_p4	CATTTCGTCGTCCAGTAGCA	GGTGATTTCTTGGTCCTCCG	50.0	55.0	58.0	58.3	1596-1612 bp	1.0	85.9
rpoA_p5	CTCGAGCCGGATGATGAAAA	GCAGGAAATGCGATTCGAAC	50.0	50.0	58.1	58.2	1952-1968 bp	1.0	85.4