Hotspot

clpP

LSC · 67036-68472 · 1437 bp

Back to Tetraena

Pi 0.0385

MarkerSeek score 69.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1437
variable_sites: 83
pi: 0.0385
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	AGCAACAAGAGCTGTATGCA	50.0	45.0	58.0	57.8	2576-2587 bp	1.0	83.2
clpP_p2	TGACCCTGTTAGTCCGTTCT	ACCAAGGCAAACCCATGAAA	50.0	45.0	58.0	58.2	2499-2510 bp	1.0	83.2
clpP_p3	TGACCCTGTTAGTCCGTTCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	2503-2514 bp	1.0	82.8
clpP_p4	TGACCCTGTTAGTCCGTTCT	CAAACGACCGGGATCATTCA	50.0	50.0	58.0	58.3	2540-2551 bp	1.0	82.6
clpP_p5	TGACCCTGTTAGTCCGTTCT	CATACCCTGCCTCCACATTG	50.0	55.0	58.0	58.3	2663-2674 bp	1.0	82.1