Hotspot

accD

LSC · 54565-55926 · 1362 bp

Back to Tetraena

Pi 0.104

MarkerSeek score 82.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1362
variable_sites: 205
pi: 0.104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AATGAGGGACGTGATCTTGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2565-2580 bp	1.0	91.2
accD_p2	AATGAGGGACGTGATCTTGC	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	2556-2571 bp	1.0	91.1
accD_p3	GTGATCTTGCTCGTGAAGGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2555-2570 bp	1.0	90.8
accD_p4	CGGTGCTGAAGATGATGGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1877 bp	0.333333	52.9
accD_p5	CGGTGCTGAAGATGATGGAA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	1868 bp	0.333333	52.7