Hotspot

trnP-UGG-psaJ

LSC · 63091-63503 · 413 bp

Back to Tetraena

Pi 0.0475

MarkerSeek score 75.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 29
pi: 0.0475
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCCACAGAGAGATATG	50.0	55.0	58.1	57.9	504 bp	1.0	83.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCCACAGAGAGATATG	50.0	55.0	58.1	57.9	503 bp	1.0	83.6
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	TAATACCGGTGCCACAGAGA	50.0	50.0	58.1	58.1	510 bp	1.0	83.5
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	TAATACCGGTGCCACAGAGA	50.0	50.0	58.1	58.1	509 bp	1.0	83.5
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	TAATACCGGTGCCACAGAGA	50.0	50.0	58.1	58.1	521 bp	1.0	83.4