Hotspot

atpB-rbcL

LSC · 51730-52534 · 805 bp

Back to Tetraena

Pi 0.0564

MarkerSeek score 78.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 67
pi: 0.0564
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGCGATATGTCCCATGTTT	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1523-1528 bp	1.0	86.3
atpB-rbcL_p2	GGGCGATATGTCCCATGTTT	TCTCTCCAACGCATAAACGG	50.0	50.0	58.0	58.0	1520-1525 bp	1.0	86.3
atpB-rbcL_p3	TTGTCAACGGGTTCTCCAAG	TCTCTCCAACGCATAAACGG	50.0	50.0	58.0	58.0	1806-1811 bp	1.0	85.5
atpB-rbcL_p4	TTGTCAACGGGTTCTCCAAG	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1809-1814 bp	1.0	85.4
atpB-rbcL_p5	AAAGCGGGTGCAGATCTATG	TCTCTCCAACGCATAAACGG	50.0	50.0	58.0	58.0	1863-1868 bp	1.0	85.4