Hotspot

trnF-GAA-trnV-UAC

LSC · 48231-48618 · 388 bp

Back to Tetraena

Pi 0.056

MarkerSeek score 81.50

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 30
pi: 0.056
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	432-455 bp	1.0	87.4
trnF-GAA-trnV-UAC_p2	AGTTCAAATCTGGTTCCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	420-443 bp	1.0	86.4
trnF-GAA-trnV-UAC_p3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	475-498 bp	1.0	85.7
trnF-GAA-trnV-UAC_p4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	474-497 bp	1.0	85.7
trnF-GAA-trnV-UAC_p5	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	431-454 bp	1.0	84.7