Hotspot

trnT-UGU-trnL-UAA

LSC · 46550-47335 · 786 bp

Back to Tetraena

Pi 0.0337

MarkerSeek score 76.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 39
pi: 0.0337
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	832-878 bp	1.0	84.4
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	CGTCTACCGATTTCGCCATA	55.0	50.0	58.2	57.6	826-872 bp	1.0	83.1
trnT-UGU-trnL-UAA_p3	AAAAGCCAGCTATCGGAGTC	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.7	58.2	847-893 bp	1.0	83.0
trnT-UGU-trnL-UAA_p4	GAAAAGCCAGCTATCGGAGT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.7	58.2	848-894 bp	1.0	83.0
trnT-UGU-trnL-UAA_p5	AAAAGCCAGCTATCGGAGTC	CGTCTACCGATTTCGCCATA	50.0	50.0	57.7	57.6	841-887 bp	1.0	81.8