Hotspot

trnR-UCU-atpA

LSC · 8643-9004 · 362 bp

Back to Tetraena

Pi 0.0534

MarkerSeek score 77.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 29
pi: 0.0534
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CGCTTTCTACTTCAGGAACA	47.6	45.0	57.3	55.4	411-425 bp	1.0	55.6
trnR-UCU-atpA_p2	GTTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACA	47.6	45.0	57.7	55.4	410-424 bp	0.666667	42.8
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACAAG	47.6	45.5	57.7	57.3	410-424 bp	0.666667	42.1
trnR-UCU-atpA_p4	GTTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACAA	47.6	42.9	57.7	56.2	410-424 bp	0.666667	41.1
trnR-UCU-atpA_p5	TTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACA	45.0	45.0	56.2	55.4	409-423 bp	0.666667	39.2