Hotspot

trnN-GUU-ndhF

IRb · 111633-113366 · 1734 bp

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Pi 0.00985

MarkerSeek score 51.80

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1734
variable_sites: 19
pi: 0.00985
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AACCCGAAATGGAAATGGCT	GGGGAAGTCGCATCTCTTTT	45.0	50.0	58.1	57.9	2329-2471 bp	1.0	83.3
trnN-GUU-ndhF_p2	GGTGACGATACTGTAGGGGA	GGGGAAGTCGCATCTCTTTT	55.0	50.0	57.9	57.9	2556-2695 bp	1.0	83.3
trnN-GUU-ndhF_p3	AACCCGAAATGGAAATGGCT	AATCCAACCCTTTCCGTCAC	45.0	50.0	58.1	58.1	2663 bp	0.333333	52.1
trnN-GUU-ndhF_p4	GGTGACGATACTGTAGGGGA	AATCCAACCCTTTCCGTCAC	55.0	50.0	57.9	58.1	2890 bp	0.333333	52.1
trnN-GUU-ndhF_p5	TTCTCTCCCACTTCACACCT	AATCCAACCCTTTCCGTCAC	50.0	50.0	57.9	58.1	2732 bp	0.333333	51.8