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clpP-psbB

LSC · 74684-75104 · 421 bp

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Pi 0.00792

MarkerSeek score 60.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 5
pi: 0.00792
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGATGGATGCTTGTC	TCAAACGACCGGGATCATTC	50.0	50.0	57.9	58.0	541-544 bp	1.0	79.5
clpP-psbB_p2	GTCAACCCAAGATGGATGCT	TCAAACGACCGGGATCATTC	50.0	50.0	57.9	58.0	545-548 bp	1.0	79.5
clpP-psbB_p3	ACCCAAGATGGATGCTTGTC	GCAACTAGGGCTGTATGCAT	50.0	50.0	57.9	58.0	575-578 bp	1.0	79.4
clpP-psbB_p4	GTCAACCCAAGATGGATGCT	GCAACTAGGGCTGTATGCAT	50.0	50.0	57.9	58.0	579-582 bp	1.0	79.4
clpP-psbB_p5	TGTCAACCCAAGATGGATGC	TCAAACGACCGGGATCATTC	50.0	50.0	58.2	58.0	546-549 bp	1.0	79.3