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psbZ-trnG-GCC

LSC · 38466-39075 · 610 bp

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Pi 0.00657

MarkerSeek score 62.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 6
pi: 0.00657
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	736-802 bp	1.0	79.2
psbZ-trnG-GCC_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	746-812 bp	1.0	78.3
psbZ-trnG-GCC_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	747-813 bp	1.0	77.9
psbZ-trnG-GCC_p4	CGGATGGTTGGTCGAGTAAC	ACATTACTTTACCCTGGCGG	55.0	50.0	58.4	57.6	775-841 bp	1.0	77.1
psbZ-trnG-GCC_p5	TTTGCTTCTCCGGATGGTTG	ACATTACTTTACCCTGGCGG	50.0	50.0	58.5	57.6	785-851 bp	1.0	76.2