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petN-psbM

LSC · 30211-31494 · 1284 bp

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Pi 0.0026

MarkerSeek score 60.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1284
variable_sites: 5
pi: 0.0026
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TGTTCGTTCTAGTTCCTACTGC	45.0	45.5	58.1	57.8	1397-1437 bp	1.0	62.1
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	TGTTCGTTCTAGTTCCTACTGC	55.0	45.5	57.9	57.8	1390-1430 bp	1.0	61.9
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	TGTTCGTTCTAGTTCCTACTGC	55.0	45.5	57.9	57.8	1389-1429 bp	1.0	61.9
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	TGTTCGTTCTAGTTCCTACTGC	55.0	45.5	57.9	57.8	1388-1428 bp	1.0	61.9
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGTTCGTTCTAGTTCCTACTGC	50.0	45.5	58.2	57.8	1435-1475 bp	1.0	61.8