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trnC-GCA-petN

LSC · 29388-30120 · 733 bp

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Pi 0.0109

MarkerSeek score 59.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 12
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.4	58.1	854-855 bp	1.0	77.5
trnC-GCA-petN_p2	ATTTTGGATCGTTTTGGCGG	ACGAGCGAAAGGGAAAATGT	45.0	45.0	57.6	58.1	893-894 bp	1.0	77.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CCATACTACGAGCGAAAGGG	50.0	55.0	58.4	57.9	861-862 bp	1.0	77.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CCCATACTACGAGCGAAAGG	50.0	55.0	58.4	57.9	862-863 bp	1.0	77.3
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CCCCATACTACGAGCGAAAG	50.0	55.0	58.4	57.9	863-864 bp	1.0	77.3