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atpH-atpI

LSC · 14501-15741 · 1241 bp

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Pi 0.00967

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 18
pi: 0.00967
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1328-1352 bp	1.0	79.8
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1384-1408 bp	1.0	79.1
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1366-1390 bp	1.0	79.0
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1329-1353 bp	1.0	78.6
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.2	57.9	1388-1412 bp	1.0	78.5