Hotspot

psbA

Genome · 66620-69350 · 2731 bp

Back to Tetradesmus

Pi 0.303
MarkerSeek score 75.40
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
Genome
length_bp
2731
variable_sites
1193
pi
0.303
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.93
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AAATCTAAGCTTGAGTTTTTCA TCTCTAAACAGTACACCGAA 27.3 40.0 52.2 53.4 2917 bp 0.2 8.0
psbA_p2 AAATCTAAGCTTGAGTTTTTCA TTCTCTAAACAGTACACCGA 27.3 40.0 52.2 53.4 2918 bp 0.2 8.0
psbA_p3 TGAAAAACTCAAGCTTAAATCT TCTCTAAACAGTACACCGAA 27.3 40.0 52.2 53.4 2933 bp 0.2 8.0
psbA_p4 TGAAAAACTCAAGCTTAAATCT TTCTCTAAACAGTACACCGA 27.3 40.0 52.2 53.4 2934 bp 0.2 8.0
psbA_p5 AAATCTAAGCTTGAGTTTTTCA TTCTCTAAACAGTACACCGAA 27.3 38.1 52.2 54.3 2918 bp 0.2 8.0