Hotspot

V9305_pgt028-V9305_pgt029

Genome · 141489-142257 · 769 bp

Back to Tetradesmus

Pi 0.382
MarkerSeek score 74.50
Primer available yes
Label V9305_pgt028-V9305_pgt029

Feature Metadata

label_name
V9305_pgt028-V9305_pgt029
feature_type
igs
region
Genome
length_bp
769
variable_sites
390
pi
0.382
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.66
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
V9305_pgt028-V9305_pgt029_p1 ATGCAGAACACAAGCACTCC ATTTTGTGGGGACAAGCCAA 50.0 45.0 58.8 58.2 468 bp 0.2 40.3
V9305_pgt028-V9305_pgt029_p2 ATGCAGAACACAAGCACTCC GTGGGGACAAGCCAAAAGAT 50.0 50.0 58.8 58.4 463 bp 0.2 39.0
V9305_pgt028-V9305_pgt029_p3 ATGCAGAACACAAGCACTCC TGGGGACAAGCCAAAAGATT 50.0 45.0 58.8 57.6 462 bp 0.2 38.8
V9305_pgt028-V9305_pgt029_p4 ATGCAGAACACAAGCACTCC TTTGTGGGGACAAGCCAAAA 50.0 45.0 58.8 58.4 466 bp 0.2 38.5
V9305_pgt028-V9305_pgt029_p5 ATGCAGAACACAAGCACTCC TTTTGTGGGGACAAGCCAAA 50.0 45.0 58.8 58.4 467 bp 0.2 38.5