Hotspot

psbM-psbZ

Genome · 108707-109506 · 800 bp

Back to Tetradesmus

Pi 0.207
MarkerSeek score 87.80
Primer available yes
Label psbM-psbZ

Feature Metadata

label_name
psbM-psbZ
feature_type
igs
region
Genome
length_bp
800
variable_sites
321
pi
0.207
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-psbZ_p1 TGCTGTTGCTGTTAATCCAA TCTTTTAGTTTTTGCAGTTGGT 40.0 31.8 55.9 55.0 882-1325 bp 1.0 52.0
psbM-psbZ_p2 TGCTGTTGCTGTTAATCCAAAG TCTTTTAGTTTTTGCAGTTGGT 40.9 31.8 57.8 55.0 882-1325 bp 1.0 52.0
psbM-psbZ_p3 TGCTGTTGCTGTTAATCCAAA TCTTTTAGTTTTTGCAGTTGGT 38.1 31.8 56.6 55.0 882-1325 bp 1.0 52.0
psbM-psbZ_p4 ATGCTGTTGCTGTTAATCCA TCTTTTAGTTTTTGCAGTTGGT 40.0 31.8 55.6 55.0 883-1326 bp 1.0 52.0
psbM-psbZ_p5 TGCTGTTGCTGTTAATCCAA TTCTTTTAGTTTTTGCAGTTGGT 40.0 30.4 55.9 55.7 883-1326 bp 1.0 52.0