Hotspot

atpB-V9305_pgt019

Genome · 104967-105741 · 775 bp

Back to Tetradesmus

Pi 0.22
MarkerSeek score 84.30
Primer available yes
Label atpB-V9305_pgt019

Feature Metadata

label_name
atpB-V9305_pgt019
feature_type
igs
region
Genome
length_bp
775
variable_sites
317
pi
0.22
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-V9305_pgt019_p1 TTTTCCTACACCAGCACCAC GGTTCGAATCCTGTCATCCC 50.0 55.0 58.0 58.0 1298-1502 bp 1.0 91.4
atpB-V9305_pgt019_p2 CAATTTTTCCACCACGACGG CAGTAGGTGCTGGTGTTGTT 50.0 50.0 58.0 58.0 1920-2377 bp 0.8 83.4
atpB-V9305_pgt019_p3 CCAATTTTTCCACCACGACG CAGTAGGTGCTGGTGTTGTT 50.0 50.0 58.0 58.0 1921-2378 bp 0.8 83.4
atpB-V9305_pgt019_p4 GCAGTTAAAGCAACACGCAT CAGTAGGTGCTGGTGTTGTT 45.0 50.0 57.9 58.0 2173-2630 bp 0.8 83.3
atpB-V9305_pgt019_p5 CAATTTTTCCACCACGACGG GGTTCGAATCCTGTCATCCC 50.0 55.0 58.0 58.0 1269-1425 bp 0.8 83.3