Hotspot

V9305_pgt014-V9305_pgt015

Genome · 91959-92502 · 544 bp

Back to Tetradesmus

Pi 0.221
MarkerSeek score 87.50
Primer available yes
Label V9305_pgt014-V9305_pgt015

Feature Metadata

label_name
V9305_pgt014-V9305_pgt015
feature_type
igs
region
Genome
length_bp
544
variable_sites
224
pi
0.221
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
V9305_pgt014-V9305_pgt015_p1 TCCTCTGCTCTACCACTGAG GAGGTGTCCTAGGCCTCTAG 55.0 60.0 57.9 58.0 605-793 bp 1.0 90.8
V9305_pgt014-V9305_pgt015_p2 TCCTCTGCTCTACCACTGAG TGAAAGGGAGGTGTCCTAGG 55.0 55.0 57.9 58.0 612-800 bp 1.0 90.5
V9305_pgt014-V9305_pgt015_p3 TCCTCTGCTCTACCACTGAG TTCGAATCCCCGTTTTCTCC 55.0 50.0 57.9 57.9 633-821 bp 1.0 90.1
V9305_pgt014-V9305_pgt015_p4 TTTGCCAGGAACCAGGATTG GAGGTGTCCTAGGCCTCTAG 50.0 60.0 58.4 58.0 649-837 bp 1.0 89.0
V9305_pgt014-V9305_pgt015_p5 TTTTGCCAGGAACCAGGATT GAGGTGTCCTAGGCCTCTAG 45.0 60.0 57.6 58.0 650-838 bp 1.0 88.7