Hotspot

rpl20-rbcL

Genome · 62126-62802 · 677 bp

Back to Tetradesmus

Pi 0.203
MarkerSeek score 84.70
Primer available yes
Label rpl20-rbcL

Feature Metadata

label_name
rpl20-rbcL
feature_type
igs
region
Genome
length_bp
677
variable_sites
250
pi
0.203
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rbcL_p1 ATAATGAAGCTGCTCCACGG CCTGGTTGTGGAGTCATACG 50.0 55.0 58.0 58.0 882-1001 bp 0.8 83.6
rpl20-rbcL_p2 ATAATGAAGCTGCTCCACGG CTGGTTGTGGAGTCATACGG 50.0 55.0 58.0 58.0 881-1000 bp 0.8 83.6
rpl20-rbcL_p3 ATAATGAAGCTGCTCCACGG GCACGAAGAGCTTTGAAACC 50.0 50.0 58.0 58.0 1137-1256 bp 0.8 83.4
rpl20-rbcL_p4 ATAATGAAGCTGCTCCACGG TCTCTCCAACGCATGAATGG 50.0 50.0 58.0 58.0 1389-1472 bp 0.6 73.9
rpl20-rbcL_p5 ATAATGAAGCTGCTCCACGG GGTCTCTCCAACGCATGAAT 50.0 50.0 58.0 58.0 1391-1474 bp 0.6 73.9