Hotspot

psbJ-psaA

Genome · 40023-40987 · 965 bp

Back to Tetradesmus

Pi 0.153

MarkerSeek score 85.60

Primer available yes

Label psbJ-psaA

Feature Metadata

label_name: psbJ-psaA
feature_type: igs
region: Genome
length_bp: 965
variable_sites: 282
pi: 0.153
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psaA_p1	ACCAAGAGCTGCTGTACCTA	ACCACACGAACGCTTAGTTT	50.0	45.0	58.1	58.1	1756-1778 bp	0.4	58.1
psbJ-psaA_p2	TACCAAGAGCTGCTGTACCT	ACCACACGAACGCTTAGTTT	50.0	45.0	58.1	58.1	1757-1779 bp	0.4	58.1
psbJ-psaA_p3	CCAAGAGCTGCTGTACCTAC	ACCACACGAACGCTTAGTTT	55.0	45.0	57.7	58.1	1755-1777 bp	0.4	56.2
psbJ-psaA_p4	ACCAAGAGCTGCTGTACCTA	GCGTATACGGTTCTGTGGAA	50.0	50.0	58.1	57.7	1283 bp	0.2	45.2
psbJ-psaA_p5	TACCAAGAGCTGCTGTACCT	GCGTATACGGTTCTGTGGAA	50.0	50.0	58.1	57.7	1284 bp	0.2	45.2