Hotspot

rps12-clpP

LSC · 69288-69468 · 181 bp

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Pi 0.0497

MarkerSeek score 76.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 9
pi: 0.0497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CGAGTACATGTTCCTCGTCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.2	57.9	346 bp	1.0	79.7
rps12-clpP_p2	CGAGTACATGTTCCTCGTCG	CGGGCAAACCTATATGGGTC	55.0	55.0	58.2	58.1	395 bp	1.0	79.5
rps12-clpP_p3	CGAGTACATGTTCCTCGTCG	GGGCAAACCTATATGGGTCG	55.0	55.0	58.2	58.1	394 bp	1.0	79.5
rps12-clpP_p4	CCGAGTACATGTTCCTCGTC	CGGGCAAACCTATATGGGTC	55.0	55.0	57.8	58.1	396 bp	1.0	79.4
rps12-clpP_p5	CCGAGTACATGTTCCTCGTC	GGGCAAACCTATATGGGTCG	55.0	55.0	57.8	58.1	395 bp	1.0	79.4