Hotspot

rpl33-rps18

LSC · 67238-67462 · 225 bp

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Pi 0.0667

MarkerSeek score 70.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 15
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACAATTCACGGGGAAATCAAG	AAAAAGTCGCTTAGATTTATTCA	42.9	26.1	56.8	52.8	276-289 bp	1.0	44.2
rpl33-rps18_p2	ACAATTCACGGGGAAATCAAGA	AAAAAGTCGCTTAGATTTATTCA	40.9	26.1	58.2	52.8	276-289 bp	1.0	44.2
rpl33-rps18_p3	ACAATTCACGGGGAAATCAAG	AGTAAAAAGTCGCTTAGATTTATTCA	42.9	26.9	56.8	55.5	279-292 bp	1.0	44.2
rpl33-rps18_p4	ACAATTCACGGGGAAATCAAG	GATTTAGTAAAAAGTCGCTTAGA	42.9	30.4	56.8	52.7	284-297 bp	1.0	44.1
rpl33-rps18_p5	ACAATTCACGGGGAAATCAAGA	GATTTAGTAAAAAGTCGCTTAGA	40.9	30.4	58.2	52.7	284-297 bp	1.0	44.1