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petA-psbJ

LSC · 62250-63187 · 938 bp

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Pi 0.0185

MarkerSeek score 71.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 17
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1125-1142 bp	1.0	70.3
petA-psbJ_p2	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1126-1143 bp	1.0	69.6
petA-psbJ_p3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.4	58.1	1099-1116 bp	1.0	69.1
petA-psbJ_p4	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.4	57.8	1100-1117 bp	1.0	68.4
petA-psbJ_p5	TCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.2	58.1	1131-1148 bp	1.0	67.6