Hotspot

psbC-trnS-UCA

LSC · 34737-34916 · 180 bp

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Pi 0.0556

MarkerSeek score 74.70

Primer available yes

Label psbC-trnS-UCA

Feature Metadata

label_name: psbC-trnS-UCA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 10
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UCA_p1	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	362-378 bp	1.0	78.7
psbC-trnS-UCA_p2	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	313-329 bp	1.0	78.7
psbC-trnS-UCA_p3	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	274-290 bp	1.0	77.6
psbC-trnS-UCA_p4	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.6	58.2	323-339 bp	1.0	77.5
psbC-trnS-UCA_p5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.5	58.2	363-379 bp	1.0	76.7