Hotspot

trnR-AGA-atpA

LSC · 9369-9663 · 295 bp

Back to Tetracme

Pi 0.0455
MarkerSeek score 75.50
Primer available yes
Label trnR-AGA-atpA

Feature Metadata

label_name
trnR-AGA-atpA
feature_type
igs
region
LSC
length_bp
295
variable_sites
13
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-AGA-atpA_p1 TCGAAAAGCGTCCATTGTCT ACATTAACCGCTGAAGCAGA 45.0 45.0 57.8 57.5 454-458 bp 1.0 78.8
trnR-AGA-atpA_p2 TCGAAAAGCGTCCATTGTCT AGACATTAACCGCTGAAGCA 45.0 45.0 57.8 57.5 456-460 bp 1.0 78.8
trnR-AGA-atpA_p3 TCGAAAAGCGTCCATTGTCT GACATTAACCGCTGAAGCAG 45.0 50.0 57.8 57.2 455-459 bp 1.0 76.0
trnR-AGA-atpA_p4 TCGAAAAGCGTCCATTGTCT TAACCGCTGAAGCAGAAAGC 45.0 50.0 57.8 58.8 450-454 bp 1.0 75.8
trnR-AGA-atpA_p5 TCGAAAAGCGTCCATTGTCT ACCAAGACATTAACCGCTGA 45.0 45.0 57.8 57.1 460-464 bp 1.0 75.7