Hotspot

trnK-AAA-rps16

LSC · 4349-4998 · 650 bp

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Pi 0.022

MarkerSeek score 70.20

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 14
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	CCGTCGATCTGTACGAATCC	CGCTCAACCAACAAGAACAG	55.0	50.0	58.0	57.6	833-837 bp	1.0	79.2
trnK-AAA-rps16_p2	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	772-776 bp	1.0	78.3
trnK-AAA-rps16_p3	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	771-775 bp	1.0	78.3
trnK-AAA-rps16_p4	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	758-762 bp	1.0	76.0
trnK-AAA-rps16_p5	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	783-787 bp	1.0	75.1