Hotspot

rpl16

LSC · 76476-77925 · 1450 bp

Back to Terniopsis

Pi 0.052

MarkerSeek score 80.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1450
variable_sites: 133
pi: 0.052
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATGGGTCTGAGGTTGGATCA	GGTGGAAAAGAAATGGCACG	50.0	50.0	58.0	57.9	1873-1886 bp	1.0	88.1
rpl16_p2	TGGGTCTGAGGTTGGATCAT	GGTGGAAAAGAAATGGCACG	50.0	50.0	58.0	57.9	1872-1885 bp	1.0	88.1
rpl16_p3	TTTCTGGCTCCGCTGTTATC	GGTGGAAAAGAAATGGCACG	50.0	50.0	58.0	57.9	1905-1918 bp	1.0	88.0
rpl16_p4	TGGGTCTGAGGTTGGATCAT	TGGGTGGAAAAGAAATGGCA	50.0	45.0	58.0	57.9	1874-1887 bp	1.0	87.9
rpl16_p5	TTTCTGGCTCCGCTGTTATC	TGGGTGGAAAAGAAATGGCA	50.0	45.0	58.0	57.9	1907-1920 bp	1.0	87.7