Hotspot

ndhF

SSC · 98689-100926 · 2238 bp

Back to Terniopsis

Pi 0.0407

MarkerSeek score 70.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 167
pi: 0.0407
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGGCTCTCATGCATTGTCT	TCAAAGCGGTCCAATAAGCT	45.0	45.0	57.3	57.5	2690-2722 bp	1.0	77.2
ndhF_p2	TGATGCGCAAACAATTTCGA	TCAAAGCGGTCCAATAAGCT	40.0	45.0	57.3	57.5	2909-2941 bp	1.0	77.0
ndhF_p3	AAGGGCAATTTCTTCTGGGT	TCAAAGCGGTCCAATAAGCT	45.0	45.0	57.3	57.5	2993-2999 bp	0.6	57.3
ndhF_p4	AGGGCAATTTCTTCTGGGTT	TCAAAGCGGTCCAATAAGCT	45.0	45.0	57.3	57.5	2992-2998 bp	0.6	57.3
ndhF_p5	GCCAGGAGCACTAAAACGAA	TCAAAGCGGTCCAATAAGCT	50.0	45.0	58.5	57.5	2760-2766 bp	0.4	48.6