Hotspot

trnP-UGG-psaJ

LSC · 63018-63484 · 467 bp

Back to Terniopsis

Pi 0.054

MarkerSeek score 84.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 45
pi: 0.054
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CTTATTACGGGTGCAACGGA	50.0	50.0	58.1	58.0	555-570 bp	1.0	87.2
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CTTATTACGGGTGCAACGGA	50.0	50.0	58.1	58.0	554-569 bp	1.0	87.2
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CTTATTACGGGTGCAACGGA	50.0	50.0	58.1	58.0	566-581 bp	1.0	87.0
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CTTATTACGGGTGCAACGGA	50.0	50.0	58.1	58.0	565-580 bp	1.0	87.0
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CTTATTACGGGTGCAACGGA	50.0	50.0	58.1	58.0	568-583 bp	1.0	86.9