Hotspot

petA-psbJ

LSC · 59726-60621 · 896 bp

Back to Terniopsis

Pi 0.0605

MarkerSeek score 81.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 106
pi: 0.0605
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTAATGTGGGTGGCTTTGGT	ACACAATCAAACCCGAACGA	50.0	45.0	58.1	58.0	1467-1509 bp	1.0	86.5
petA-psbJ_p2	CTAATGTGGGTGGCTTTGGT	TTCGTCAGCACTGGATTAGC	50.0	50.0	58.1	58.0	1769-1811 bp	1.0	85.7
petA-psbJ_p3	TAATGTGGGTGGCTTTGGTC	TTCGTCAGCACTGGATTAGC	50.0	50.0	58.1	58.0	1768-1810 bp	1.0	85.7
petA-psbJ_p4	CTAATGTGGGTGGCTTTGGT	CGTCAGCACTGGATTAGCTT	50.0	50.0	58.1	58.0	1767-1809 bp	1.0	85.7
petA-psbJ_p5	TAATGTGGGTGGCTTTGGTC	CGTCAGCACTGGATTAGCTT	50.0	50.0	58.1	58.0	1766-1808 bp	1.0	85.7