Hotspot

trnQ-UUG-trnK-UUU

LSC · 50535-51366 · 832 bp

Back to Terniopsis

Pi 0.0808

MarkerSeek score 87.70

Primer available yes

Label trnQ-UUG-trnK-UUU

Feature Metadata

label_name: trnQ-UUG-trnK-UUU
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 118
pi: 0.0808
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-trnK-UUU_p1	TAAGGCAACGGGTTTTGGTC	AGTCGCACTTAAAAGCCGAG	50.0	50.0	58.7	58.6	859-931 bp	1.0	82.0
trnQ-UUG-trnK-UUU_p2	TAAGGCAACGGGTTTTGGTC	TAGTCGCACTTAAAAGCCGA	50.0	45.0	58.7	57.3	860-932 bp	1.0	80.7
trnQ-UUG-trnK-UUU_p3	TAAGGCAACGGGTTTTGGTC	TCGCACTTAAAAGCCGAGTA	50.0	45.0	58.7	57.3	857-929 bp	1.0	80.7
trnQ-UUG-trnK-UUU_p4	TAAGGCAACGGGTTTTGGTC	CGCACTTAAAAGCCGAGTAC	50.0	50.0	58.7	57.2	856-928 bp	1.0	80.3
trnQ-UUG-trnK-UUU_p5	TAAGGCAACGGGTTTTGGTC	GTCGCACTTAAAAGCCGAGT	50.0	50.0	58.7	58.9	858-930 bp	1.0	79.7