Hotspot

trnR-UCU-trnG-UCC

LSC · 47118-47783 · 666 bp

Back to Terniopsis

Pi 0.0697

MarkerSeek score 85.70

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 81
pi: 0.0697
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	AAATCCTGCGTCCAATAGGA	AGCCTTCCAAGCTAACGATG	45.0	50.0	56.6	58.0	632-833 bp	1.0	80.6
trnR-UCU-trnG-UCC_p2	AAATCCTGCGTCCAATAGGA	CCTAGCCTTCCAAGCTAACG	45.0	55.0	56.6	58.1	635-836 bp	1.0	80.4
trnR-UCU-trnG-UCC_p3	ATCCTGCGTCCAATAGGATT	AGCCTTCCAAGCTAACGATG	45.0	50.0	56.4	58.0	630-831 bp	1.0	78.7
trnR-UCU-trnG-UCC_p4	AAAATCCTGCGTCCAATAGGA	AGCCTTCCAAGCTAACGATG	42.9	50.0	57.4	58.0	633-834 bp	1.0	78.6
trnR-UCU-trnG-UCC_p5	ATCCTGCGTCCAATAGGATT	CCTAGCCTTCCAAGCTAACG	45.0	55.0	56.4	58.1	633-834 bp	1.0	78.5