Hotspot

atpI-atpH

LSC · 42573-43110 · 538 bp

Back to Terniopsis

Pi 0.0486

MarkerSeek score 81.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 45
pi: 0.0486
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	ATGGAAGGGCATCATTGA	AGCGGCAGAAATCAATGGAT	44.4	45.0	53.3	57.9	570-826 bp	1.0	48.4
atpI-atpH_p2	ATGGAAGGGCATCATTGA	AAGCGGCAGAAATCAATGGA	44.4	45.0	53.3	58.2	571-827 bp	1.0	48.4
atpI-atpH_p3	ATGGAAGGGCATCATTGA	GAAGCGGCAGAAATCAATGG	44.4	50.0	53.3	57.8	572-828 bp	1.0	48.4
atpI-atpH_p4	ATGGAAGGGCATCATTGA	ATAACGGAAGCGGCAGAAAT	44.4	45.0	53.3	58.0	578-834 bp	1.0	48.3
atpI-atpH_p5	ATGGAAGGGCATCATTGA	AATAACGGAAGCGGCAGAAA	44.4	45.0	53.3	58.2	579-835 bp	1.0	48.3