Hotspot

petN-trnC-GCA

LSC · 28340-29141 · 802 bp

Back to Terniopsis

Pi 0.0569

MarkerSeek score 82.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 85
pi: 0.0569
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	GGGGGACTGCAAATCCTTTT	50.0	50.0	58.2	58.4	874-888 bp	1.0	84.3
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	849-863 bp	1.0	79.0
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	851-865 bp	1.0	78.5
petN-trnC-GCA_p4	GCCCAAGCGAGACTTACTAT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.1	58.4	873-887 bp	1.0	78.4
petN-trnC-GCA_p5	ATTAATGCAGCCCAAGCGAG	GGGGGACTGCAAATCCTTTT	50.0	50.0	59.0	58.4	882-896 bp	1.0	77.7