Hotspot

trnL-UAA-trnT-UGU

LSC · 10976-11491 · 516 bp

Back to Terniopsis

Pi 0.0628
MarkerSeek score 83.00
Primer available yes
Label trnL-UAA-trnT-UGU

Feature Metadata

label_name
trnL-UAA-trnT-UGU
feature_type
igs
region
LSC
length_bp
516
variable_sites
60
pi
0.0628
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnT-UGU_p1 GCGTCTACCTATTTCGCCAT GCGATGCTCTAACCTCTGAG 50.0 55.0 57.8 57.9 561-594 bp 1.0 87.7
trnL-UAA-trnT-UGU_p2 GCGTCTACCTATTTCGCCAT GAGTCGAACCGATGACCATC 50.0 55.0 57.8 58.2 592-625 bp 1.0 87.3
trnL-UAA-trnT-UGU_p3 GCGTCTACCTATTTCGCCAT CTAACCTCTGAGCTAAGCGG 50.0 55.0 57.8 57.5 553-586 bp 1.0 84.8
trnL-UAA-trnT-UGU_p4 GCGTCTACCTATTTCGCCAT ATGCGATGCTCTAACCTCTG 50.0 50.0 57.8 57.5 563-596 bp 1.0 84.6
trnL-UAA-trnT-UGU_p5 GCGTCTACCTATTTCGCCAT TGCGATGCTCTAACCTCTGA 50.0 50.0 57.8 58.5 562-595 bp 1.0 84.5