Hotspot

rbcL-atpB

LSC · 3534-4398 · 865 bp

Back to Terniopsis

Pi 0.05

MarkerSeek score 79.70

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 78
pi: 0.05
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	GCCTTGAATCCAACACTTGC	TTCAAGTGCGGAAACTCCAA	50.0	45.0	57.9	58.0	953-959 bp	1.0	88.1
rbcL-atpB_p2	GCCTTGAATCCAACACTTGC	AAGTGCGGAAACTCCAAGAC	50.0	50.0	57.9	58.4	950-956 bp	1.0	85.3
rbcL-atpB_p3	GCCTTGAATCCAACACTTGC	CAAGTGCGGAAACTCCAAGA	50.0	50.0	57.9	58.4	951-957 bp	1.0	85.2
rbcL-atpB_p4	GCCTTGAATCCAACACTTGC	TCAAGTGCGGAAACTCCAAG	50.0	50.0	57.9	58.4	952-958 bp	1.0	85.2
rbcL-atpB_p5	TATACTCTTTAACGCCGGCC	TTCAAGTGCGGAAACTCCAA	50.0	45.0	57.5	58.0	970-976 bp	1.0	84.4