Hotspot

rps15

SSC · 120782-121060 · 279 bp

Back to Tephroseris

Pi 0.00358

MarkerSeek score 40.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 1
pi: 0.00358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGAGGTAAATCAGCAGCGAC	AAGAACCAGAATGCTGTGCA	50.0	45.0	58.0	58.0	1327-1340 bp	1.0	80.7
rps15_p2	AACACCATGCATTGATGGGT	AAGAACCAGAATGCTGTGCA	45.0	45.0	58.1	58.0	896-909 bp	1.0	80.7
rps15_p3	CCTAATTGCTCTGGCCCATT	AAGAACCAGAATGCTGTGCA	50.0	45.0	57.9	58.0	1090-1103 bp	1.0	80.4
rps15_p4	TGAGGTAAATCAGCAGCGAC	AATTATGGAACCTGCCCGTC	50.0	50.0	58.0	57.9	1610-1623 bp	1.0	80.3
rps15_p5	TGAGGTAAATCAGCAGCGAC	TATGGAACCTGCCCGTCTAT	50.0	50.0	58.0	57.9	1607-1620 bp	1.0	79.9