Hotspot

rpl16

LSC · 79759-81200 · 1442 bp

Back to Tephroseris

Pi 0.000704

MarkerSeek score 60.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1442
variable_sites: 1
pi: 0.000704
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCGATTTTTGAGGCCAATT	AGTTTCTTCTTTTTCTTTTCTGTTCA	45.0	26.9	58.0	56.4	1546-1566 bp	1.0	41.1
rpl16_p2	CGCGATTTTTGAGGCCAATT	GTTTCTTCTTTTTCTTTTCTGTTCA	45.0	28.0	58.0	55.2	1545-1565 bp	1.0	41.1
rpl16_p3	CGCGATTTTTGAGGCCAATT	TCTTCTTTTTCTTTTCTGTTCA	45.0	27.3	58.0	52.1	1542-1562 bp	1.0	41.1
rpl16_p4	CGCGATTTTTGAGGCCAATT	AAGTTTCTTCTTTTTCTTTTCTGTTCA	45.0	25.9	58.0	57.0	1547-1567 bp	1.0	41.1
rpl16_p5	CGCGATTTTTGAGGCCAATT	AGTTTCTTCTTTTTCTTTTCTGTTCAA	45.0	25.9	58.0	57.0	1546-1566 bp	1.0	41.1