Hotspot

ndhF-rpl32

SSC · 109825-110846 · 1022 bp

Back to Tephroseris

Pi 0.00295

MarkerSeek score 60.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1022
variable_sites: 3
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACAAAGCTTTTAACGCTGCC	50.0	45.0	58.0	57.9	1217-1230 bp	1.0	79.1
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	1206-1219 bp	1.0	79.1
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1215-1228 bp	1.0	79.0
ndhF-rpl32_p4	TTGCCGTCGGAAAAAGAAGA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1216-1229 bp	1.0	79.0
ndhF-rpl32_p5	TACTTTTTGTTGCCGTCGGA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1225-1238 bp	1.0	79.0