Hotspot

trnT-GGU-psbD

LSC · 31034-32253 · 1220 bp

Back to Tephroseris

Pi 0.00167

MarkerSeek score 61.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 2
pi: 0.00167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TACAGTGACCCGTCAGGAAT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1403-1415 bp	1.0	80.6
trnT-GGU-psbD_p2	TAACTCAGCGGTAGAGCACT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.2	58.0	1357-1369 bp	1.0	79.9
trnT-GGU-psbD_p3	TAGTACAGTGACCCGTCAGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.2	58.0	1406-1418 bp	1.0	79.8
trnT-GGU-psbD_p4	TACAGTGACCCGTCAGGAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1421-1433 bp	1.0	79.7
trnT-GGU-psbD_p5	GTGACCCGTCAGGAATTGAA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1399-1411 bp	1.0	79.7