Hotspot

trnC-GCA-petN

LSC · 9493-10295 · 803 bp

Back to Tephroseris

Pi 0.00249

MarkerSeek score 60.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 2
pi: 0.00249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	916 bp	1.0	78.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	859 bp	1.0	78.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	877 bp	1.0	76.1
trnC-GCA-petN_p4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.3	58.2	917 bp	1.0	73.6
trnC-GCA-petN_p5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.0	57.1	915 bp	1.0	72.7