Hotspot

rpoC2

LSC · 26653-31227 · 4575 bp

Back to Temochloa

Pi 0.00445

MarkerSeek score 59.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4575
variable_sites: 30
pi: 0.00445
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CTGGTCGAATTAGGGGAAGC	TCGATTCAACAAGGTGCCAA	55.0	45.0	58.0	58.0	2124-2268 bp	1.0	84.3
rpoC2_p2	CTGGTCGAATTAGGGGAAGC	TTCGATTCAACAAGGTGCCA	55.0	45.0	58.0	58.0	2125-2269 bp	1.0	84.3
rpoC2_p3	CAACCATTTCGAGCACAACC	TCGATTCAACAAGGTGCCAA	50.0	45.0	57.9	58.0	2229-2373 bp	1.0	84.3
rpoC2_p4	CAACCATTTCGAGCACAACC	TTCGATTCAACAAGGTGCCA	50.0	45.0	57.9	58.0	2230-2374 bp	1.0	84.3
rpoC2_p5	TACGCGAGGGACTTTCTTTG	TCGATTCAACAAGGTGCCAA	50.0	45.0	57.9	58.0	2564-2708 bp	1.0	84.0