Hotspot

rbcL

LSC · 56579-58012 · 1434 bp

Back to Temochloa

Pi 0.00139

MarkerSeek score 41.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 3
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGTAGACCCTGTCGTTGTGA	AGCCGAGTTTAATTGCAATCA	50.0	38.1	58.0	56.5	1608 bp	1.0	60.1
rbcL_p2	ATCGAGTAGACCCTGTCGTT	AGCCGAGTTTAATTGCAATCA	50.0	38.1	57.9	56.5	1612 bp	1.0	59.2
rbcL_p3	TAGACCCTGTCGTTGTGAGA	AGCCGAGTTTAATTGCAATCA	50.0	38.1	57.7	56.5	1606 bp	1.0	58.0
rbcL_p4	AGTAGACCCTGTCGTTGTGA	AGCCGAGTTTAATTGCAATCAA	50.0	36.4	58.0	57.2	1608 bp	1.0	57.8
rbcL_p5	GTAGACCCTGTCGTTGTGAG	AGCCGAGTTTAATTGCAATCA	55.0	38.1	57.7	56.5	1607 bp	1.0	57.4