Hotspot

petA

LSC · 61651-62613 · 963 bp

Back to Temochloa

Pi 0.00208

MarkerSeek score 60.10

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 3
pi: 0.00208
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	ATTCTGGGATCTGCGATTGG	GCCAATAAATCGCGGCTTTT	50.0	45.0	57.7	58.1	1035 bp	1.0	77.5
petA_p2	ATTCTGGGATCTGCGATTGG	CAATCGCCAATAAATCGCGG	50.0	50.0	57.7	58.3	1040 bp	1.0	75.5
petA_p3	ATTCTGGGATCTGCGATTGG	GATGGTAAGAAATCCCGGGC	50.0	55.0	57.7	58.4	1006 bp	1.0	74.9
petA_p4	ATTCTGGGATCTGCGATTGG	AGCAATCGCCAATAAATCGC	50.0	45.0	57.7	57.6	1042 bp	1.0	74.8
petA_p5	TTCTGGGATCTGCGATTGGA	GCCAATAAATCGCGGCTTTT	50.0	45.0	58.8	58.1	1034 bp	1.0	73.2