Hotspot

ndhH

SSC · 116557-117738 · 1182 bp

Back to Temochloa

Pi 0.00226

MarkerSeek score 60.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 4
pi: 0.00226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCAGTAGGAGGGTCAAAA	TTGATGAGCCTCGTTTTGAG	50.0	45.0	57.6	56.1	1370 bp	1.0	61.4
ndhH_p2	TCCCAGTAGGAGGGTCAAAA	TTTGATGAGCCTCGTTTTGAG	50.0	42.9	57.6	56.8	1371 bp	1.0	59.4
ndhH_p3	TCCCAGTAGGAGGGTCAAAA	TTTGATGAGCCTCGTTTTGA	50.0	40.0	57.6	55.6	1371 bp	1.0	57.5
ndhH_p4	TCCCAGTAGGAGGGTCAAAA	TTTTGATGAGCCTCGTTTTGAG	50.0	40.9	57.6	57.5	1372 bp	1.0	56.9
ndhH_p5	TCCCAGTAGGAGGGTCAAAAT	TTGATGAGCCTCGTTTTGAG	47.6	45.0	58.1	56.1	1370 bp	1.0	56.2