Hotspot

matK

LSC · 1680-3215 · 1536 bp

Back to Temochloa

Pi 0.00217

MarkerSeek score 60.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 5
pi: 0.00217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAGAATGGAAAACGTGAAAGT	CAACACCCTGTTCTGACCAT	36.4	50.0	56.5	57.7	1699 bp	1.0	49.9
matK_p2	TCAGAATGGAAAACGTGAAAGT	AATGCAACACCCTGTTCTGA	36.4	45.0	56.5	57.6	1703 bp	1.0	49.2
matK_p3	TCAGAATGGAAAACGTGAAAGT	ATGCAACACCCTGTTCTGAC	36.4	50.0	56.5	58.4	1702 bp	1.0	49.0
matK_p4	ACATTTCAGAATGGAAAACGTGA	CAACACCCTGTTCTGACCAT	34.8	50.0	57.2	57.7	1704 bp	1.0	47.9
matK_p5	AGGGAACATTTCAGAATGGA	CAACACCCTGTTCTGACCAT	40.0	50.0	54.2	57.7	1709 bp	1.0	47.6