Hotspot

infA

LSC · 78461-78802 · 342 bp

Back to Temochloa

Pi 0.00195

MarkerSeek score 30.40

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 342
variable_sites: 1
pi: 0.00195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	CGCAAACCAATACGCACTTT	TTAAAGCGTATCAGCCGACC	45.0	50.0	58.0	58.1	1074-1075 bp	1.0	79.8
infA_p2	TCCACTACCAGCACCCTTTA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1336-1337 bp	1.0	79.7
infA_p3	TTGCAAACCCACTGTACGAA	TTAAAGCGTATCAGCCGACC	45.0	50.0	58.0	58.1	1300-1301 bp	1.0	79.7
infA_p4	GTAATGCGGCATCTCTTCCA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1352-1353 bp	1.0	79.6
infA_p5	TAATGCGGCATCTCTTCCAC	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1351-1352 bp	1.0	79.6