Hotspot

ndhF-rpl32

SSC · 107114-107882 · 769 bp

Back to Temochloa

Pi 0.0078

MarkerSeek score 51.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 769
variable_sites: 9
pi: 0.0078
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGACAAAATACTGGAATTCTTCA	TGACATCGAAGTACGTTTTT	30.4	35.0	53.9	53.2	824-827 bp	1.0	41.1
ndhF-rpl32_p2	AGACAAAATACTGGAATTCTTCA	TTGACATCGAAGTACGTTTT	30.4	35.0	53.9	53.2	825-828 bp	1.0	41.1
ndhF-rpl32_p3	AGACAAAATACTGGAATTCTTCA	TTTGACATCGAAGTACGTTT	30.4	35.0	53.9	53.2	826-829 bp	1.0	41.1
ndhF-rpl32_p4	AGACAAAATACTGGAATTCTTCA	TTTTGACATCGAAGTACGTT	30.4	35.0	53.9	53.2	827-830 bp	1.0	41.1
ndhF-rpl32_p5	AGACAAAATACTGGAATTCTTCA	TCCAAAGATTTTTACGAATACGCT	30.4	33.3	53.9	57.3	852-855 bp	1.0	41.0