Hotspot

rpl14-rpl16

LSC · 79840-79948 · 109 bp

Back to Temochloa

Pi 0.0306

MarkerSeek score 72.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 5
pi: 0.0306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCAGCTCCTATGACTCGAA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.2	58.2	252 bp	1.0	77.8
rpl14-rpl16_p2	CGAGCTCCACTGTTATCTGC	TAGCTGCCAGTAAAATGCCC	55.0	50.0	58.4	58.2	218 bp	1.0	76.5
rpl14-rpl16_p3	TAGCAGCTCCTATGACTCGA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.4	58.2	253 bp	1.0	74.6
rpl14-rpl16_p4	AGCAGCTCCTATGACTCGAA	GCCAGTAAAATGCCCATACG	50.0	50.0	58.2	57.2	247 bp	1.0	73.6
rpl14-rpl16_p5	GCAGCTCCTATGACTCGAAT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.2	58.2	251 bp	1.0	73.2